Lunate excision, capitate osteotomy, and intercarpal arthrodesis should be used with caution for advanced Kienböck’s disease

Liposome-mediated RNA transfection should be used with caution.

Liposome-mediated RNA transfection appears to present a number of advantages for studying the metabolism of reporter mRNAs in mammalian cells. This method is also widely used to transfect siRNAs. Here we describe results indicating that reporter mRNAs introduced into HeLa cells by liposomes do not present the expected behaviors. Namely, the stability of reporter mRNAs was independent of the pre...

Transfection of microRNA Mimics Should Be Used with Caution

Transient transfection of chemically synthesized microRNA (miRNA) mimics is being used extensively to study the functions and mechanisms of endogenous miRNAs. However, it remains unclear whether transfected miRNAs behave similarly to endogenous miRNAs. Here we show that transient transfection of miRNA mimics into HeLa cells by a commonly used method led to the accumulation of high molecular wei...

receiver operating characteristic curve and odds ratio should be used with caution

Three-corner midcarpal arthrodesis and scaphoidectomy: a simplified volar approach.

Midcarpal arthrodesis has been a treatment of choice in the last decade for scapholunate advanced collapse and related conditions of the wrist. In this study, we present a new uncomplicated technique in which a 3-corner intercarpal fusion is done with screw fixation from a volar approach. The advantages of this technique include simplified excision of the scaphoid, radial styloidectomy, and str...

Receiver Operating Characteristic Curve and Odds Ratio Should Be Used with Caution

We read with interest the article by Abu El Makarem MA et al. (1) entitled, “Platelet count/bipolar spleen diameter ratio for the prediction of esophageal varices: The special Egyptian situation.” This study evaluated noninvasive predictors of esophageal varices in cirrhotic patients by multivariate logistic regression and receiver operating characteristic (ROC) curve analysis. However, in our ...